An hormonal influence on calcium transport was evident in (i) the decrease in specific radioactivity of rabbit blood plasma with advancing pregnancy, (ii) the extraordinarily rapid calcium transport between blood plasma and endometrium, especially in the oestrous stage, and (iii) the effectiveness of ovarian hormone substitution in ovariectomized rabbits. A marked downward gradient in values of flux existed between the progestational endometrium, uterine fluid and blastocyst there was a similar gradient between placental tissues and foetus. Oestrous endometrium had the largest rapidly exchanging calcium fraction (turnover time of 12min) and the highest value for calcium flux (500μg of Ca exchanged/h per g fresh wt.
Compartmental analysis was applied to measurements of specific radioactivity. Marked variability in calcium content characterized rabbit tissues and body fluids. The following tissues were examined at short- (45min and 2h) and long-duration (4, 16 and 48h) intervals after parenteral administration of 45Ca or 47Ca: maternal blood plasma, endometrium, uterine fluid, placental tissues, two developmentally disparate stages of rabbit conceptus, namely the unattached blastocyst and the early post-implantation foetus, and bone (femur). The kinetics of calcium transport were studied in unmated (oestrous) and pregnant rabbits in the first half of gestation, with the aim of establishing evidence of hormonal ( ovarian) influence on the pattern of transport. All rights reserved.Ĭalcium transport in the early conceptus and associated maternal tissues in the rabbitġ. Although the role of FXR in female reproductive tissues is still not known, it is possible to consider various functions related to the reproductive tissue. The uterine epithelium and musculature of virgins had scarce anti-FXR immunoreactivity. Also, oviductal and vaginal epithelium of virgins, as well as the oviductal smooth muscle, showed anti-FXR immunoreactivity. FXR immunoreactivity was found in all types of ovarian follicles, ovarian stroma, and corpus luteum of virgin and pregnant rabbits. Immunohistochemistry for FXR was done and reproductive tissues were photographed. Rabbits were sacrificed and their reproductive tissues were excised and histologically processed.
Considering that these events occur in reproductive tissues in females, the aim of the present study was to determine the immunolocalization of FXR in the ovary, oviduct, uterus, and vagina of rabbits. Farnesoid X receptor immunolocalization in reproductive tissues of adult female rabbits.Īnaya-Hernández, Arely Méndez-Tepepa, Maribel Hernández-Aragón, Laura G Pacheco, Pablo MartÃnez-Gómez, Margarita Castelán, Francisco Cuevas, Estelaįarnesoid X receptor (FXR) has been involved in lipid metabolism, cell proliferation, apoptosis, and aromatase expression, as well as in the steroid synthesis and signaling.
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